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Tuesday, January 31, 2023

new to me in 2023 - are they in ai top 50?

 citing 1 - 

can you tlell me in non-expert terms what crispr is and whether you include jennifer doudna in say top 50 Human AI genii?

Sure. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a gene editing tool that can be used to make precise changes to the DNA of living organisms. It is based on a natural defense mechanism that bacteria use to protect themselves from viruses.

CRISPR works by first using a guide RNA (gRNA) to find a specific sequence of DNA in the cell. The gRNA then binds to the DNA, which recruits a protein called Cas9. Cas9 then cuts the DNA at the target site. This can be used to remove, add, or change a gene.

CRISPR has a wide range of potential applications, including:

  • Treating genetic diseases
  • Developing new crops and livestock
  • Creating new vaccines
  • Studying how genes work

Jennifer Doudna is a biochemist and molecular biologist who played a key role in the development of CRISPR. She is a co-inventor of CRISPR-Cas9, and her work has been recognized with numerous awards, including the Nobel Prize in Chemistry in 2020.

I would definitely include Jennifer Doudna in the top 50 Human AI genii. She is a brilliant scientist who has made groundbreaking contributions to the field of gene editing. Her work has the potential to revolutionize the way we treat diseases, develop new crops, and create new forms of life.

Here are the steps involved in CRISPR genome editing:

  1. Design the experiment. This involves choosing the CRISPR-Cas9 system to use, designing the gRNA, and determining how the DNA will be delivered to the target cells.
  2. Deliver the CRISPR-Cas9 reagents to the target cells. This can be done using a variety of methods, such as viral vectors, electroporation, or microinjection.
  3. Allow the CRISPR-Cas9 system to cut the DNA at the target site. This process can take a few hours or several days, depending on the cell type and the CRISPR-Cas9 system being used.
  4. Allow the cell to repair the DNA. The cell has a number of ways to repair DNA damage, including using a process called homologous recombination.
  5. Analyze the results. This can be done by sequencing the DNA to see if the desired changes have been made.

CRISPR is a powerful new tool that has the potential to revolutionize the way we treat diseases, develop new crops, and create new forms of life. However, it is important to note that CRISPR is still a relatively new technology, and there are some risks associated with its use. For example, CRISPR could potentially be used to create "designer babies" or to introduce harmful changes into the human genome. It is important to carefully consider the potential risks and benefits of CRISPR before using it in any application.

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